SC 7 - Sample Preparation for Bioanalytical LC-MS

Prof. Dr. Dr. Karl-Siegfried Boos and
Dr. Rosa Morello, Institute of Clinical Chemistry, Medical Center of the University of Munich, Germany

Course contents:
This short course is dedicated to technicians and chemists responsible for method development, analytical chemists, laboratory supervisors and scientists in the biochemical, pharmaceutical, clinical and environmental setting but also in other fields. It will cover instrumentation, operational procedures and practical guidelines for integration of solid-phase extraction (SPE) into LC-systems coupled to tandem mass spectrometry. A focus of this course will also be the principles and application of multidimensional SPE. Properties and performance of tailor-made SPE packing materials (e.g. restricted access materials, RAM, molecularly imprinted polymers, MIP, mixed-mode materials such as Oasis, etc.) as well as high throughput and miniaturization of SPE-LC will be discussed in detail. Besides direct injection and on-line SPE of urine and plasma samples, the direct injection and in-line processing of whole blood will be addressed. A general introduction to the understanding, monitoring and elimination of ion suppression / matrix effects in bioanalytical LC-MS/MS will be given. Finally, a broad spectrum of applications in the fields of therapeutic drug monitoring, biological monitoring, environmental – and clinical-chemical analysis will be presented.

Instructors:
Karl-Siegfried Boos: 1968 - 1977 Technical and Medical University, Hannover, Germany; 1974 Graduation in Biochemistry; 1977 Academic degree: Dr. rer. nat; 1977 - 1978 Department of Biochemistry, Washington State University, USA; 1979 - 1990 Laboratory of  Biological Chemistry, University of Paderborn; 1987 Professor of Biological Chemistry. Since 1990 Head of the Laboratory  of BioSeparation at the Institute of Clinical Chemistry, Medical Center of the Ludwig-Maximilians University (LMU), Munich; 1994 Professor of Experimental Clinical Chemistry and academic degree Dr. rer. biol. hum. Over 100 original publications in the field of Biochemistry, Analytical Chemistry and Medicine. Since 2000 co-editor of the journal “Chromatographia”.

Rosa Morello: 1995 - 2000 Student of Pharmacy at the Universities of Würzburg and Munich; 2000 -2001 Practical year and Approbation as Pharmacist; 2001 - 2004 Ph.D. student of Prof. Dr. Dr. Karl-Siegfried Boos, Laboratory of BioSeparation, Medical Center of the Ludwig-Maximilians University (LMU), Munich; 2007 Doctoral degree Dr. rer. biol. hum. from the Medical Faculty of the LMU; 2004-to date Senior research scientist at the same laboratory. Expert for preanalytics and integrated clean-up of complex (bio)fluids as well as their on-line analysis with high  performance separation and detection platforms (e.g. SPE-LC-MS/MS). Presentation of numerous basic and advanced courses on this topic.