SC 6 - How to Set up and Optimize Multidimensional Liquid Chromatography Separations

Dr. Martin Gilar, Waters Corporation, Milford, MA, USA
Prof. Dr. Peter Schoenmakers, Free University of Amsterdam, Amsterdam, The Netherlands

Course contents:
This half day course will introduce attendees into the principles of two-dimensional liquid chromatography (2-D LC) and multidimensional experiments. Participants will learn about options for the selection of suitable stationary phases, useful column dimensions, and the intricacies of 2-D LC experimental setup. The instructors will describe the types of experiments, beginning with implementation of column switching, off-line 2-D LC, and ending with comprehensive on-line 2-D LC. Attendees will learn about peak capacity and separation orthogonality concepts and their implications for multidimensional separations. Specific application examples will be given for proteomic and peptidomic applications, polymer separation, chiral analysis, and others. We will compare 2-D LC applications in terms of implementation difficulty, mobile phase compatibility limitations, separation mode options, and suitable detection techniques. Traditional as well as novel/unusual 2-D LC separation modes will be discussed.

Instructors:
Martin Gilar received his Ph.D. in analytical chemistry from the Institute of Chemical Technology in Prague (1996). He spent postdoc years at Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and a fraction collector for DNA molecules. Since 1998 he works at Waters Corporation in Milford, Massachusetts where he is a principal researcher in the Biopharmaceutical Sciences department. He has more than ten years of experience in the separation sciences, namely chromatography, electrophoresis, and mass spectrometry. His research interests are sample preparation and analysis of biomolecules, such as oligonucleotides, peptides and proteins.

Peter Schoenmakers studied chemistry at the Technical University of Delft and received a Ph.D. in 1982 for a thesis entitled "A systematic approach to mobile-phase effects in reversed-phase liquid chromatography". He held a number of industrial occupations, including the Philips Research Laboratories, Eindhoven, and the Shell Laboratorium, Amsterdam. Since 1998 he is a professor and group leader of the polymer-analysis group at the University of Amsterdam, The Netherlands.  His primary expertise is liquid chromatography (HPLC), furthermore supercritical-fluid chromatography (SFC), gas chromatography (GC), method development and optimization, as well as polymer separations. Other expertise is mass spectrometry, forensic analysis, oil and petrochemistry.