SC 5 - Supercritical Fluid Chromatography and Green Chemistry in the Pharmaceutical Industry

Prof. Dr. Larry Taylor, Virginia Tech, Blacksburg, VA, USA
Dr. Larry Miller, Amgen Inc., Cambridge, MS, USA

Course contents:
This course will focus on the fundamentals and advances in supercritical fluid chromatography (SFC) employing carbon dioxide-based mobile phases and packed column stationary phases. Particular emphasis will be directed toward pharmaceutical and other industrial fields where SFC plays a critical role. The course will cover:

• Fundamental properties of supercritical fluids
• Analytical and preparative scale separations
• Advantages of carbon dioxide-based mobile phases relative to conventional liquid mobile phase solvents in terms of: 1. column efficiency per unit time 2. sample throughput 3. cost of analysis/purification 4. detector compatibility and interface design 5. multiple stacked columns for enhanced resolution 6. hyphenation with sample preparation
• Guidelines for stationary phase selection
• Comparison of SFC with normal phase HPLC for both achiral and chiral analytes
• Detection with emphasis on mass spectrometry, light scatter and UV
• Method development for chiral SFC and mechanisms of separation
• Instrumentation for preparative scale separations in conjunction with high throughput requirements
• Fundamental physico-chemical properties of compressible fluid phases within the context of chromatographic science
• Role of both mobile phases modifiers and additives in the separation of polar analytes: mobile phase vs. stationary phase
• Applications that incorporate a wide variety of functional groups relevant to pharmaceutical science such as quaternary amine salts, phospholipids, vitamins, nucleosides, polypeptides, polyphenols, and surfactants
• A unified chromatographic approach in which solvating power, diffusivity, and viscosity are variables
• SFC directly coupled with sample preparation and other chromatographies

Instructors:
Larry T. Taylor holds a BS degree in Chemistry from Clemson University (1962) and obtained a doctoral degree in 1965. He joined the Virginia Tech faculty as an Assistant Professor in 1967 after spending two and one-half years as a National Institutes of Health Postdoctoral Fellow at the Ohio State University working on the synthesis and properties of metal complexes containing macrocyclic ligands related to biological materials. Dr. Taylor was promoted to Associate Professor in 1970 and Professor in 1978. He has authored over 350 refereed technical publications.

Larry Miller is a senior scientist in the Discovery Analytical Sciences group at Amgen in Cambridge, MA.  He graduated with a BS degree from the University of Illinois in Urbana-Champaign in 1984 and a MS from Roosevelt University in 1990. In 1984 he joined Searle in suburban Chicago as an analytical chemist.  He spent twenty years performing small molecule achiral and chiral purifications at the mg to multi-kg scale, utilizing preparative HPLC, steady state recycle (SSR) and simulated moving bed (SMB) chromatography.  He managed a group of ten scientists within a purification center of excellence at Searle/Pharmacia, providing purification support to three R&D sites.  In 2004 Larry joined Amgen. At Amgen he is responsible for discovery and early development purification support utilizing preparative SFC and HPLC.